Journal: Data in Brief

Article Title: Data on 2D culture characterisation of potential markers in human HER2-positive breast cancer cell lines

doi: 10.1016/j.dib.2022.108880

Figure Lengend Snippet: Gene expression profile of marker panel in basal condition (80% confluence) in two HER2-positive BC cell lines. The marker panel included genes in SKBR3 cultures (A) and in MDA-MB-453 cultures (B): E-Cadherin ( CDH1 ), P-Cadherin ( CDH3 ), p21-cyclin dependent kinase inhibitor 1A ( CDKN1A ), p16-cyclin dependent kinase inhibitor ( CDKN2A ), Wingless-related integration site 1 ( WNT1 ), Wingless-related integration site 4 ( WNT4 ), Cytokeratin 4 ( CK4 ),Cytokeratin 8 ( CK8 ), Cytokeratin 18 ( CK18 ), Cytokeratin 19 ( CK19 ), Axin-1 ( AXIN1 ), Axin-2 ( AXIN2 ), Smooth muscle Actin Alpha 2 ( ACTA2 ), Cyclin-dependent kinase 2 ( CDK2 ), Nuclear Catenin B1 ( CTNNB1 ), Fibroblast growth factor receptor 1 ( FRFR1 ), MAP3K5 Mitogen-activated protein kinase 5 ( ASK1 ). Graph bars = relative gene expression, all error bars are presented as standard deviation (SD).

Article Snippet: How the data were acquired , SKBR3 and MDA-MB-453 cell lines were obtained from ATCC. SKBR3 (1 × 1e4 cells/cm 2 ) and MDA-MB-453 (2 × 1e4 cells/cm 2 ) cells were grown as a monolayer under basal growth conditions (80% confluence). Total RNA was extracted by Direct-zolTM RNA MiniPrep kit (Zymo Research Corp., USA), then converted to complementary DNA (cDNA) using iScript (BioRad). The cDNA template was the utilised to perform Q-PCR using the QuantStudio 7 Flex Real-Time PCR system (Thermo Fisher Scientific)..

Techniques: Gene Expression, Marker, Standard Deviation

Journal: Data in Brief

Article Title: Data on 2D culture characterisation of potential markers in human HER2-positive breast cancer cell lines

doi: 10.1016/j.dib.2022.108880

Figure Lengend Snippet: Average cell number of HER2-positive breast cancer cells treated with varying concentrations (0–50 µg/ml) of heparin from day 1 (D1) to day 7 (D7). Cells were plated at 3000 cells/well for SKBR3, and 1 × 1e4 cells/well for MDA-MB-453, and in 24 well-plates. Total live cell count was assessed in cells collected at D1, D3, D5 and D7 of SKBR3 and MDA-MB-453 cultures treated with 0 µg/ml, 1 µg/ml, 5 µg/ml, 10 µg/ml, 25 µg/ml, and 50 µg/ml. Error bar = Standard deviation (SD).

Article Snippet: How the data were acquired , SKBR3 and MDA-MB-453 cell lines were obtained from ATCC. SKBR3 (1 × 1e4 cells/cm 2 ) and MDA-MB-453 (2 × 1e4 cells/cm 2 ) cells were grown as a monolayer under basal growth conditions (80% confluence). Total RNA was extracted by Direct-zolTM RNA MiniPrep kit (Zymo Research Corp., USA), then converted to complementary DNA (cDNA) using iScript (BioRad). The cDNA template was the utilised to perform Q-PCR using the QuantStudio 7 Flex Real-Time PCR system (Thermo Fisher Scientific)..

Techniques: Cell Counting, Standard Deviation

Journal: Data in Brief

Article Title: Data on 2D culture characterisation of potential markers in human HER2-positive breast cancer cell lines

doi: 10.1016/j.dib.2022.108880

Figure Lengend Snippet:

Article Snippet: How the data were acquired , SKBR3 and MDA-MB-453 cell lines were obtained from ATCC. SKBR3 (1 × 1e4 cells/cm 2 ) and MDA-MB-453 (2 × 1e4 cells/cm 2 ) cells were grown as a monolayer under basal growth conditions (80% confluence). Total RNA was extracted by Direct-zolTM RNA MiniPrep kit (Zymo Research Corp., USA), then converted to complementary DNA (cDNA) using iScript (BioRad). The cDNA template was the utilised to perform Q-PCR using the QuantStudio 7 Flex Real-Time PCR system (Thermo Fisher Scientific)..

Techniques: Real-time Polymerase Chain Reaction, Extraction, Gene Expression, Control, Generated, Software

Journal: International Journal of Medical Sciences

Article Title: Effect of SDF-1 and CXCR4 gene variants on the development of diabetic kidney disease

doi: 10.7150/ijms.103186

Figure Lengend Snippet: Impact of rs1801157 and rs266085 genotypes on SDF-1 expression. Comparisons of SDF-1 (CXCL12) expression among (A) rs1801157 and (B) rs266085 genotypic groups in representative normal tissues based on data from the GTEx portal. p values were calculated among groups by one-way ANOVA.

Article Snippet: Biallelic discrimination for rs1801157 (assay ID: C_3223115_10, rs2297630 (assay ID: C_3223122_1), rs2839693 (assay ID: C_31777299_10), rs266085 (assay ID: C_1033724_30), rs2228014 (assay ID: C_27378716_10) and rs6430612 (assay ID: C_30721949_10) SNPs was carried out through the TaqMan assay (Applied Biosystems, Foster City, CA, USA), and genotypes were determined by SDS version 3.0 software.

Techniques: Expressing